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| PEREIRA,GUILLERMO; ALBORNOZ,VERÓNICA; ROMERO,CHRISTIAN; LARA,SEBASTIÁN; SÁNCHEZ-OLATE,MANUEL; RÍOS,DARCY; ATALA,CRISTIAN. |
| ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark at 24±1 ºC for 2 weeks. Then they were put in 16/8 h light/dark cycles for 14 weeks. We... |
| Tipo: Journal article |
Palavras-chave: Terrestrial orchids; Endemic species; In vitro germination; Culture media; Orchid propagation; Asymbiotic germination. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131 |
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| Rodrigues,Bruno Rafael Alves; Santos,Rayane Carneiro dos; Nietsche,Silvia; Mercadante-Simões,Maria Olívia; Cunha,Isabella Renata Gomes da; Pereira,Marlon Cristian Toledo. |
| ABSTRACT The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro germination; Pollen viability; Monads; Tetrads; Pollen tube. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000200145 |
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| SANTA-CATARINA,CLAUDETE; MACIEL,SCHEILA DA CONCEIÇÃO; PEDROTTI,ENIO LUIZ. |
| Embriões zigóticos em diferentes estádios de desenvolvimento foram utilizados para avaliar o potencial de germinação e competência embriogênica in vitro. Frutos coletados entre 15 e 360 dias após a antese foram avaliados em relação ao diâmetro (mm), comprimento (mm) e massa fresca (g). Para avaliar a percentagem de germinação in vitro, embriões zigóticos em diferentes estádios de desenvolvimento foram inoculados em meio de cultura composto de sais minerais de Murashigue & Skoog (MS), ou em meio MS suplementado com 4,4 miM de BAP, sacarose (3%), ágar (0,6%), carvão ativado (0,15%). Para a indução de embriogênese somática, eixos embrionários em diferentes estádios de desenvolvimento foram inoculados em meio de cultura MS, suplementado com 2,4-D (0,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zygotic embryos; Somatic embryogenesis; In vitro germination; Woody plant; Lauraceae. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500004 |
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| Diamantino, Maria Selma Alves Silva; Costa, Maria Angélica Pereira de Carvalho; Soares, Taliane Leila; Morais, Daniel Vieira; Silva, Simone Alves; Souza, Everton Hilo de. |
| The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L.) and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%). For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fitotecnia Ricinus communis L.; In vitro germination; Colorimetric analysis; Pollen tube Melhoramento Vegetal. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25981 |
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| Soares,Taliane Leila; Jesus,Onildo Nunes de; Souza,Everton Hilo de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de. |
| The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro germination; Histochemistry; Scanning electron microscopy; Passifloraceae; Pollen tube. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018 |
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| Amorim,Juliane dos Santos; Souza,Margarete Magalhães; Viana,Américo José Carvalho; Freitas,Jôsie Cloviane de Oliveira. |
| This study aimed to characterize the reproductive system of Passiflora capsularis L. and P. rubra L. In vivo controlled pollinations, in vitro pollen germination and pollen-ovule (P:O) ratio evaluation were conducted. In self-pollination, intraspecific and interspecific pollination, P. capsularis showed means of 62.5, 68.7 and 48.4% of fertilized flowers, while in P. rubra, the averages were 67.2, 62.5 and 46.9%, respectively. For in vitro germination, 52.2% of P. capsularis pollen grains germinated while in P. rubra, the percentage was 64.4. The P:O ratio was 22.4 for P. capsularis, and 27.4 for P. rubra, which included them in the category of obligatory autogamous. Passiflora capsularis and P. rubra can reproduce both by self-pollination and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vivo pollination; In vitro germination; Passion flower; Pollen-ovule ratio. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000400007 |
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